Botulinum toxin A is perpetually used in medical treatment for curing muscle spasm since Botox can cause muscle relaxation. Later, Botox is developed and used in cosmetic surgery. Botox initial substance consists of active substance (Botulinum) and binding substance (protein). In the past, it is believed that when inject any substance to your body, protein will coat the active substance to prevent damage from body. Actually, however, protein itself stimulates antibody causing Botox resistance. Therefore, first injection is fine, but other injections give less results or nothing. Moreover, injection to a wrong area or an inadequate dose can cause Botox resistance as well.